Composite

Part:BBa_K1017101:Design

Designed by: Wen-Hui Cheng   Group: iGEM13_NCTU_Formosa   (2013-09-12)

Pred ( PompC + B0032 + LacI + J61048 + Plac)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1282
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1296
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since the transcription factor of PompC, OmpR, is phosphorylated in dark and non phosphorylated in red light, we used LacI and Plac downstream PompC to reverse the original function of PompC. By this way, we can express the genes downstream in red light and block them in dark, making this biobrick a red light-induced promoter.


Source

Assemble parts form iGEM distribution

References